解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
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神经系统科学
护理
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产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
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药理学和药剂学
生理学
基本医疗保健
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公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
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风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract:: ...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-018-0270-1
更新日期:2019-01-01 00:00:00
abstract::Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. This method allows for near-native...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-018-0238-1
更新日期:2019-01-01 00:00:00
abstract::Preprocessing of functional magnetic resonance imaging (fMRI) involves numerous steps to clean and standardize the data before statistical analysis. Generally, researchers create ad hoc preprocessing workflows for each dataset, building upon a large inventory of available tools. The complexity of these workflows has s...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-018-0235-4
更新日期:2019-01-01 00:00:00
abstract::An outstanding challenge of epigenome-wide association studies (EWASs) performed in complex tissues is the identification of the specific cell type(s) responsible for the observed differential DNA methylation. Here we present a statistical algorithm called CellDMC ( https://github.com/sjczheng/EpiDISH ), which can ide...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-018-0213-x
更新日期:2018-12-01 00:00:00
abstract:: ...
journal_title:Nature methods
pub_type: 评论,杂志文章
doi:10.1038/s41592-018-0248-z
更新日期:2018-12-01 00:00:00
abstract:: ...
journal_title:Nature methods
pub_type: 社论
doi:10.1038/s41592-018-0257-y
更新日期:2018-12-01 00:00:00
abstract:: ...
journal_title:Nature methods
pub_type: 评论,杂志文章
doi:10.1038/s41592-018-0200-2
更新日期:2018-11-01 00:00:00
abstract::FLIRT (fast local infrared thermogenetics) is a microscopy-based technology to locally and reversibly manipulate protein function while simultaneously monitoring the effects in vivo. FLIRT locally inactivates fast-acting temperature-sensitive mutant proteins. We demonstrate that FLIRT can control temperature-sensitive...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-018-0168-y
更新日期:2018-11-01 00:00:00
abstract:: ...
journal_title:Nature methods
pub_type: 评论,新闻
doi:10.1038/s41592-018-0192-y
更新日期:2018-11-01 00:00:00
abstract::Sequencing of RNA 3' ends has uncovered numerous sites that do not correspond to the termination sites of known transcripts. Through their 3' untranslated regions, protein-coding RNAs interact with RNA-binding proteins and microRNAs, which regulate many properties, including RNA stability and subcellular localization....
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-018-0114-z
更新日期:2018-10-01 00:00:00
abstract::In the originally published paper, the "before" image for the afatinib condition in Fig. 6c was incorrect. Instead of an image displaying a GBM-3 neoplastic organoid before afatinib treatment, this panel showed an image from the GBM-2 control (DMSO) group before treatment. This error has now been corrected in the HTML...
journal_title:Nature methods
pub_type: 已发布勘误
doi:10.1038/s41592-018-0118-8
更新日期:2018-09-01 00:00:00
abstract::Despite widespread use of CRISPR, comprehensive data on the frequency and impact of Cas9-mediated off-targets in modified rodents are limited. Here we present deep-sequencing data from 81 genome-editing projects on mouse and rat genomes at 1,423 predicted off-target sites, 32 of which were confirmed, and show that hig...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-018-0011-5
更新日期:2018-07-01 00:00:00
abstract::Thus far, optical recording of neuronal activity in freely behaving animals has been limited to a thin axial range. We present a head-mounted miniaturized light-field microscope (MiniLFM) capable of capturing neuronal network activity within a volume of 700 × 600 × 360 µm3 at 16 Hz in the hippocampus of freely moving ...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/s41592-018-0008-0
更新日期:2018-06-01 00:00:00
abstract::MicroRNAs (miRNAs) play an essential role in the post-transcriptional regulation of animal development and physiology. However, in vivo studies aimed at linking miRNA function to the biology of distinct cell types within complex tissues remain challenging, partly because in vivo miRNA-profiling methods lack cellular r...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4610
更新日期:2018-04-01 00:00:00
abstract::Single-molecule localization microscopy techniques have proven to be essential tools for quantitatively monitoring biological processes at unprecedented spatial resolution. However, these techniques are very low throughput and are not yet compatible with fully automated, multiparametric cellular assays. This shortcomi...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4486
更新日期:2017-12-01 00:00:00
abstract::The stable expansion of tissue-specific stem cells in vitro has contributed to research on several organs. Alveolar epithelial type II (AT2) cells function as tissue stem cells in the lung, but robust models for studying human AT2 cells are lacking. Here we report a method for the efficient generation and long-term ex...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4448
更新日期:2017-11-01 00:00:00
abstract::We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4396
更新日期:2017-10-01 00:00:00
abstract::The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4294
更新日期:2017-07-01 00:00:00
abstract::The normalization of RNA-seq data is essential for accurate downstream inference, but the assumptions upon which most normalization methods are based are not applicable in the single-cell setting. Consequently, applying existing normalization methods to single-cell RNA-seq data introduces artifacts that bias downstrea...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4263
更新日期:2017-06-01 00:00:00
abstract::Ribonucleoproteins (RNPs) are key regulators of cellular function. We established an efficient approach, crosslinking of segmentally isotope-labeled RNA and tandem mass spectrometry (CLIR-MS/MS), to localize protein-RNA interactions simultaneously at amino acid and nucleotide resolution. The approach was tested on pol...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4235
更新日期:2017-05-01 00:00:00
abstract::High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 150 neurons in the hippocampal...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4183
更新日期:2017-04-01 00:00:00
abstract::We present single-cell interpretation via multikernel learning (SIMLR), an analytic framework and software which learns a similarity measure from single-cell RNA-seq data in order to perform dimension reduction, clustering and visualization. On seven published data sets, we benchmark SIMLR against state-of-the-art met...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4207
更新日期:2017-04-01 00:00:00
abstract::CRISPR-based genetic screens are accelerating biological discovery, but current methods have inherent limitations. Widely used pooled screens are restricted to simple readouts including cell proliferation and sortable marker proteins. Arrayed screens allow for comprehensive molecular readouts such as transcriptome pro...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4177
更新日期:2017-03-01 00:00:00
abstract::The ability to selectively interfere with post-translationally modified proteins would have many biological and therapeutic applications. However, post-translational modifications cannot be selectively targeted by nucleic-acid-based interference approaches. Here we describe post-translational intracellular silencing a...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4144
更新日期:2017-03-01 00:00:00
abstract::Recent work has demonstrated that environmental factors experienced by parents can affect their offspring across multiple generations, and that such transgenerational transmission can depend on the germline. Causal evidence for the involvement of germ cells is rare, however, and the underlying molecular mechanisms rem...
journal_title:Nature methods
pub_type: 杂志文章,评审
doi:10.1038/nmeth.4181
更新日期:2017-02-28 00:00:00
abstract::Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation ...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4178
更新日期:2017-02-13 00:00:00
abstract::The mechanical properties of the cellular microenvironment and their spatiotemporal variations are thought to play a central role in sculpting embryonic tissues, maintaining organ architecture and controlling cell behavior, including cell differentiation. However, no direct in vivo and in situ measurement of mechanica...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4101
更新日期:2017-02-01 00:00:00
abstract::Gene expression is extensively regulated at the levels of mRNA stability, localization and translation. However, decoding functional RNA-regulatory features remains a limitation to understanding post-transcriptional regulation in vivo. Here, we developed RNA-element selection assay (RESA), a method that selects RNA el...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4121
更新日期:2017-02-01 00:00:00
abstract::Structure probing coupled with high-throughput sequencing could revolutionize our understanding of the role of RNA structure in regulation of gene expression. Despite recent technological advances, intrinsic noise and high sequence coverage requirements greatly limit the applicability of these techniques. Here we desc...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4068
更新日期:2017-01-01 00:00:00
abstract::We report an atmospheric pressure (AP) matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) setup with a lateral resolution of 1.4 μm, a mass resolution greater than 100,000, and accuracy below ±2 p.p.m. We achieved this by coupling a focusing objective with a numerical aperture (NA) of ...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4071
更新日期:2017-01-01 00:00:00
abstract::A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new re...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.4045
更新日期:2016-12-01 00:00:00
abstract::Phenotype-based small-molecule screening is a powerful method to identify molecules that regulate cellular functions. However, such screens are generally performed in vitro under conditions that do not necessarily model complex physiological conditions or disease states. Here, we use molecular cell barcoding to enable...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3992
更新日期:2016-10-01 00:00:00
abstract::Stable in vivo mapping and modulation of the same neurons and brain circuits over extended periods is critical to both neuroscience and medicine. Current electrical implants offer single-neuron spatiotemporal resolution but are limited by such factors as relative shear motion and chronic immune responses during long-t...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3969
更新日期:2016-10-01 00:00:00
abstract::We have developed hydrogel-based virtual microfluidics as a simple and robust alternative to complex engineered microfluidic systems for the compartmentalization of nucleic acid amplification reactions. We applied in-gel digital multiple displacement amplification (dMDA) to purified DNA templates, cultured bacterial c...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3955
更新日期:2016-09-01 00:00:00
abstract::The ability to image RNA identity and location with nanoscale precision in intact tissues is of great interest for defining cell types and states in normal and pathological biological settings. Here, we present a strategy for expansion microscopy of RNA. We developed a small-molecule linker that enables RNA to be cova...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3899
更新日期:2016-08-01 00:00:00
abstract::We present a method to robustly discriminate clustered from randomly distributed molecules detected with techniques based on single-molecule localization microscopy, such as PALM and STORM. The approach is based on deliberate variation of labeling density, such as titration of fluorescent antibody, combined with quant...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3897
更新日期:2016-08-01 00:00:00
abstract::Accurate identification of cell subsets in complex populations is key to discovering novelty in multidimensional single-cell experiments. We present X-shift (http://web.stanford.edu/~samusik/vortex/), an algorithm that processes data sets using fast k-nearest-neighbor estimation of cell event density and arranges popu...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3863
更新日期:2016-06-01 00:00:00
abstract::Counting molecules in complexes is challenging, even with super-resolution microscopy. Here, we use the programmable and specific binding of dye-labeled DNA probes to count integer numbers of targets. This method, called quantitative points accumulation in nanoscale topography (qPAINT), works independently of dye phot...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3804
更新日期:2016-05-01 00:00:00
abstract::Small molecules are known to stabilize membrane proteins and to modulate their function and oligomeric state, but such interactions are often hard to precisely define. Here we develop and apply a high-resolution, Orbitrap mass spectrometry-based method for analyzing intact membrane protein-ligand complexes. Using this...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3771
更新日期:2016-04-01 00:00:00
abstract::To enable the detection of expression signatures specific to individual cells, we developed PLAYR (proximity ligation assay for RNA), a method for highly multiplexed transcript quantification by flow and mass cytometry that is compatible with standard antibody staining. When used with mass cytometry, PLAYR allowed for...
journal_title:Nature methods
pub_type: 杂志文章
doi:10.1038/nmeth.3742
更新日期:2016-03-01 00:00:00